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Olympus BX43 Fluorescence Microscope
Product Overview
The Olympus BX43 is an advanced research-grade microscope designed for transmitted light (Brightfield) and fluorescence imaging. It combines high optical performance, modular flexibility, and upgradeability, making it widely used in research, diagnostic, and educational laboratories.
The BX43 is commonly applied in cell and molecular biology, genetics, pathology, cytogenetics, microbiology, and medical research.
This system enables users to observe natural cellular structures under brightfield illumination and to detect and image fluorescently labeled molecules with high clarity and precision.
Modular and Professional Design
The Olympus BX43 features a fully modular and flexible design, allowing laboratories to configure the system according to their specific needs.
Fluorescence components, filter sets, light sources, cameras, and imaging software can be added or upgraded without replacing the entire microscope.
BX43 is ideal for laboratories seeking a combination of professional performance and easy upgradeability.
Fluorescence Filter Sets (Channels)
The BX43 supports multiple fluorescence filter cubes (typically 3–6 positions in the filter turret) for multi-channel fluorescence imaging.
Common filter sets include:
DAPI (Blue) – for nuclear staining
FITC / GFP (Green)
TRITC / Cy3 (Orange-Red)
Texas Red / mCherry
Cy5 / Alexa Fluor 647
Optional filters for CFP and YFP
🔹 These filters allow simultaneous detection of multiple biomarkers within a single sample.
Light Sources
The BX43 can be equipped with different light sources depending on the configuration:
For Brightfield Imaging:
Halogen lamp with adjustable intensity
LED illumination with uniform, natural color
For Fluorescence Imaging:
Mercury lamp
Metal halide light sources
Next-generation LED fluorescence illumination
These options provide efficient excitation across a wide spectral range, from DAPI to Cy5.
Special Application: FISH (Fluorescence In Situ Hybridization)
The Olympus BX43 is suitable for FISH (Fluorescence In Situ Hybridization) applications.
In FISH, fluorescent probes are used to detect and localize specific DNA or RNA sequences within cells or chromosomes.
BX43 is ideal for FISH due to:
High-resolution, precise optics
Stable and uniform fluorescence illumination
Accurate fluorescence filter sets
Multi-channel imaging capability
It can be used effectively for:
Cytogenetic analysis
Detection of chromosomal abnormalities
Cancer diagnostics and genetic mutation screening
Infertility and IVF research
Key Features
1. Multi-Channel Fluorescence Imaging
Simultaneous visualization of multiple fluorescent probes in a single specimen.
2. Camera & Software Connectivity
Compatible with CCD and CMOS cameras for:
High-resolution image capture
FISH documentation
Quantitative image analysis using software such as ImageJ or cellSens
3. Professional Optical Quality
Olympus objectives provide:
Exceptional resolution
High contrast
Accurate color reproduction
4. Upgradeable and Flexible Platform
Expandable with:
Additional fluorescence filters
Advanced LED or metal halide light sources
Digital imaging systems
Applications
FISH and cytogenetics
Cell and molecular biology
Medical genetics
Pathology and histology
Cancer research
Research, diagnostic, and educational laboratories
